A study on the secondary renal injury and the change of TNF-α and IL-1β of traumatic hemorrhagic shock in dry-heat desert and normal temperature environment
Li Jian-ying
Liu Jiang-wei
An Zong-ren
Li Xin-yu
Shen Cai-fu
Duan Yue
Wei Lian-qun
Abstract：Objective To research the relationship between the secondary renal injury and the change of inflammatory cytokines TNF-α and IL-1β in dry-heat and normal temperature environment.Methods 48 healthy Landrace pigs were randomly divided into the dry-heat experimental (DHS)group and normal temperature experimental (NTS) group.The model of traumatic hemorrhagic shock was established after 3 hours exposure in the dry-heat environment.After the model was successfully divided into three groups according to different time points:T1 (model successfully 50 min,n =6),T2 (moel successfully 100 min,n =6),T3 (model successfully 150 min,n =6).Normal temperature group exposed to ambient temperature 3 hours and after the model was successfully divided into five groups according to different time point:T1 (model successfully 50 min,n =6),T2 (model successfully 100 min,n =6),T3 (model successfully 150 min,n =6),T4 (model successfully 480 min,n =6),T5 (model successfully 600 min,n =6).To collect blood urea nitrogen and creatinine;collect renal tissue to detect inflammatory cytokines TNF-α and IL-1β and the morphology change of renal glomerular and renal tubular and Paller score at each time point.Results There was no significant difference (P ＞ 0.05) in blood urea nitrogen and creatinine between the NTS group and the DHS group at T1 time point,and there was significant difference (P ＜ 0.05) at T2 and T3 time points.At T1,T2 and T3 time point,Paller score,TNF-α and IL-1β were significantly higher in DHS group than those in NTS group (P ＜ 0.05).Correlation analysis showed that TNF-oα and Paller score had positive correlation (rNTs =0.863,rDHS =0.905,P ＜ 0.01).IL--1β was positively correlated with Paller score (rNTs =0.823,rDHs =0.946,P ＜ 0.01).At the same time,the levels of blood urea nitrogen,creatinine,Paller score,TNF-α and IL-1β in DHS group were significantly higher than those in NTS group at T3 time point (P ＜ 0.05).Conclusion When dry-heat environment combined with traumatic hemorrhagic shock happens,the indexes of renal injury (blood urea nitrogen and creatinine),Paller score and inflammatory cytokines (TNF-α and IL-1β) are significantly higher than those in traumatic hemorrhagic shock group,indicating that the dry-heat environment and traumatic hemorrhagic shock co-driven the expression of inflammatory factor may play an important role in secondary renal injury.
Keywords：Dry-heat environmentTraumatic hemorrhagic shock (THS)Kidney injuryInflammatory factor
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 265-269,封4 )