Effect of lipopolysaccharide on the expression of VWF and ADAMTS13 in rat pulmonary micro-vascular endothelial cells and the intervention of vitamin D
Xu Jun
Yang Jia-lai
Chen Jian
Luo Qing-li
Meng Ling-yi
Yu Feng
Zhang Hong
Abstract：Objective To observe the effects of lipopolysaccharide(LPS)on cultured rat pul?monary microvascular endothelial cells(PMVEC)von Willebrand factor(VWF), A disintegrin- like andmetalloproteinase with thrombospond in type-1 domain 13(ADAMTS13)expression, and the inter?vention effect of vitamin D(Vit D). Methods Primary cultured rat PMVEC were randomly assigned to 6 groups: NC group, LPS group(given 100 μg /mL LPS)and calcitriol(Cal)group(given 100 nmol/L Cal), LPS+Cal 1, 2, 3 groups(treated with 100 μg /mL LPS and 5 nmol/l, 20 nmol/L, 100 nmol/L Cal) were used to detect the expression of VWF and ADAMTS13 mRNA and protein in cell lysates. Results VWF, ADAMTS13 mRNA and protein expression showed no significant changes in LPS group and Cal group, VWF mRNA and protein expression levels in LPS group and LPS + Cal groups were significantly higher than those in NC group; VWF mRNA and protein expression levels in LPS + Cal groups of were significantly lower than those in LPS group; ADAMTS13 mRNA and protein expression levels in LPS group and LPS + Cal groups were significantly lower than those in NC group; ADAMTS13 mRNA and protein expression levels in LPS+Cal 3 group were significantly higher than those in the LPS group. Con-clusion Vit D in physiological condition does not change the expression of VWF and ADAMTS13 in PMVECs, while after being stimulated by LPS Vit D can prevent the increase of VWF expression and the reduction of ADAMTS13 caused by LPS. In the pathogenesis of sepsis or ALI, Vit D may play a role in the disease outcome by affecting the expression of VWF and ADAMTS13 in PMVEC.
Keywords：Lipopolysaccharide(LPS)Pulmonary microvascular endothelial cells(PMVEC)Von Willebrand FactorA disintegrin-like andmetalloproteinase with thrombos-pond in type-1 domain 13(ADAMTS13)Vitamin D(Vit D)
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1036-1039,1040 )