Effect of propofol pretreatment on endoplasmic reticulum stress in rat brain after cardiopulmo-nary resuscitation
Ge Dong
Xie Xue-meng
Xu Jian-feng
Zhang Jin-cheng
Wu Yan-li
Yang Guang-tian
Abstract：Objective To explore the effect of propofol pretreatment on postresuscitation brain protection in a rat model of cardiac arrest and the relationship with endoplasmic reticulum stress(ERS). Methods Forty-five male SD rats were randomly divided into Sham group(n=15), Intralipid group (n=15)and Propofol group(n=15). Rats of Sham group conducted all operations without induce of cardiac arrest. Rats of Intralipid group and Propofol group were operated to induce cardiac arrest by transcutaneous electrical stimulation to epicardium. In Propofol group, propofol(10 mg/kg)was adminis? trated via the intraperitoneal 30 min before operation. In Intralipid group, MCT/LCT(same volume of propofol)was administrated via the intraperitoneal 30 min before operation. The survival rats were ob?served 24 h after restoration of spontaneous circulation(ROSC)and neurological status was evaluated by neurological deficit score(NDS). 24 h after ROSC, brains were removed for analysis of hematoxylin-eo?sin(HE)staining, TUNEL assays and western-blot. Results Compared with the Sham group, the rats in Intralipid group and Propofol group have poorer neurological outcomes(P<0.05), varying degrees histopathological changes in the CA1 region of the hippocampus and higher apoptosis index(P<0.01). Compared with the Intralipid group, there was a significant improvement in NDS(P<0.05), histopatho?logical changes and apoptosis index(P<0.01)in Propofol group. The expressive protein of GRP78, XBP-1, CHOP and caspase12 in Intralipid group and Propofol group were higher than those in Sham group(P< 0.01). Compared with the Intralipid group, the Propofol group had lower expressive protein of XBP-1, CHOP and caspase12(P<0.01), but higher expression of GRP78 protein(P<0.01). Con-clusion Propofol pretreatment can mitigate brain injury after ROSC through inhibition of ERS.
Keywords：Cardiac arrest(CA)Cardiopulmonary resuscitation(CPR)PropofolEndo-plasmic reticulum stress(ERS)
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1031-1035 )