Effects of hydrogen sulfide on rats hypothalamic-pituitary-adrenal axis response during sepsis
Peng Chao
Qiao Wei
Pang Zhong-duo
Yue Jian-yun
Qi Ying-wen
Lu Yan
Chai Chen
Abstract：Objective To investigate the effects of hydrogen sulfide(H2S)on serum corticoste?rone(CORT), adrenocorticotropin(ACTH)and hypothalamic corticosterone(CRH)concentration in septic rats. Methods Sepsis was induced in adult male Sprague-Dawley(SD)rats by cecal ligation and puncture(CLP). Sixty-three rats were randomly divided into nine groups: sham operation group;sepsis 6 h, 12 h, 24 h groups; CLP 12 h + sodium hydrosulfide(NaHS); CLP 12 h + hydroxylamine (HA)+ propargylglycine(PAG)group; CLP 12 h+ HA group; CLP 12 h+ PAG group(n=7 in every group). CLP only rats were sacrificed at 6 h, 12 h, 24 h after CLP. The others received intraperitoneal in?jection of different drugs 1 h before CLP and were sacrificed at 12 h after CLP. The samples of serum or hypothalamus were collected. Serum CORT, ACTH and hypothalamic CRH concentration were detected by enzyme-linked immunosorbent assay(ELISA). Results ①Serum CORT, ACTH and hypothalamic CRH concentrations increased significantly 6 h, 12 h, 24 h after CLP(all P<0.05). Serum CORT, ACTH and hypothalamic CRH concentrations were significantly higher 12 h, 24 h after CLP than that of 6 h after CLP(all P<0.05). Serum CORT concentration at 24 h after CLP was significantly higher than that 12 h after CLP(P<0.05), serum ACTH concentration was significantly lower than that 24 h after CLP(P<0.05). ②Inhibition of central or peripheral H2S generation increased serum CORT, ACTH and hypothalamic CRH concentrations(all P<0.05). Inhibition of the production of central H2S increased serum CORT, ACTH and hypothalamic CRH concentration(all P<0.05). Inhibition of the production of peripheral H2S increased serum CORT concentration(P<0.05)and decreased hypothalamic CRH concentration(P<0.05). Serum ACTH concentration did not significantly changed(P<0.05). Con-clusion HPA axis is active during sepsis; H2S inhibit HPA axis response by central and peripheral mechanism during sepsis.
Keywords：SepsisHypothalamic- pituitary- adrenal axis(HPA axis)Hydrogen sulfide (H2S)
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 975-979 )