The correlation between CTGF and liver fibrosis after paraquat poisoning in rats
LI Zeng-pan
FANG Jian-jiang
XIA Jing
LIU Xue-lan
ZHOU Ting
Abstract：Objective To investigate connective tissue growth factor( CTGF) in the model rats with lung fibrosis after paraquat poisoning. Methods Fifty-four male SD rats were randomly divided into three groups,control group( NS group) , paraquat group( PQ group) , Fasudil group( FS group) . Rats were exposed to 50 mg/kg paraquat by oral gavage for establishing the animal model of paraquat poisoning. The rats in NS group were given equal normal saline. FS group was treated with Fasudil through intraperitoneal injection at 30 mg/(kg·d). The rats were sacrificed on 7,14,28 days after poinsoning. The lung tissue was harvested to HE staining and Masson staining. Lung fibrosis was assessed by Ashcroft score. Real-time PCR was used to test the content of CTGF mRNA in the lung tissues of the rats of three groups. Results The Ashcroft score of lung tissue and CTGF mRNA in lung of PQ group were higher than those in NS group(P<0. 05). While the Ashcroft score of lung tissue and CTGF mRNA in lung of PQ group were lower than those in PQ group(P<0. 05). The CTGF mRNA was associated with increased Ashcroft scores(Spearman r =0. 089,P <0. 01). Conclusion CTGF expression is increased significantly in the lung tissues after paraquat poisoning. The expression of CTGF is related with the degree of lung fibrosis.
Keywords：Connective tissue growth factor(CTGF)Paraquat poisoningLung fibrosis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 565-568 )