Effect of tea polyphenols on brain neurology cell apoptosis in rats with cardiac arrest following cardio-pulmonary resuscitation
WANG HUI-hui
RUANSHI Fang-ying
CHEN Meng-hua
SHU Quan
XIE Lu
Abstract：Objective To study the effect of tea polyphenols ( TP) on nerve cell apoptosis in rats with cardiac arrest following cardio-pulmonary resuscitation ( CPR ) . Methods Healthy SD rats were randomly divided into sham ( SH) group, normal saline ( NS) group, and tea polyphenols ( TP) group. In addition to the SH group, others were induced ventricular fibrillation esophagus stimulation with alternating current. Five min after cardiac arrest CPR was performed. Once restoration of spontaneous circulation ( ROSC) was gained, TP or NS were immediately administrated. Cortex and hippocampus of rats in each group were collected at 12, 24, 48, 72 h after ROSC. TUNEL( TdT mediated dUTP Nick end labeling) and caspase 3 fluorescence intensity, as well as caspase 12 expression of Western blotting at each time point were measured respectively. Results TUNEL and caspase 3 fluorescence density at 72 h of TP group and NS group were greater than SH group (P<0. 05), while the TUNEL and caspase 3 fluorescence density of TP group were smaller than that of NS group (P < 0. 05). Caspase 12 expression of TP at 48, 72 h decreased significantly when compared with NS group and SH group ( P<0. 05), and the expression of NS group was higher than that in SH group (P<0. 05). Conclusion TP can inhibit nerve cell apoptosis caused by ischemia-reperfusion after CPR, which relates to decreasing the activation of caspase 3 and caspase 12.
Keywords：Cardio-pulmonary resuscitationCardiacarrest(CA)Ischemia reperfusionCell apoptosis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 552-555 )