The effect of vitamin D on the expression of serum vWF and ADAMTS13 in sepsis rat model
XU Jun
YANG Jia-lai
CHEN Jian
CHENG Li-shun
YANG Jing
MENG Ling-yi
YU Feng
ZHANG Hong
Abstract：Objective To observe the effect of vitamin D( VD) on the expression of serum von Willebrand factor( vWF ) and adisintegrin -like and metalloproteinase with thrombospond in type -1 domain 13 ( ADAMTS13 ) in sepsis rat model. Methods Sepsis rat model was prepared by intraperitoneal injection of lipopolysaccharide method, and randomly divided to 5 groups:normal control group, sepsis group and calcitriol group (which were treated with calcitriol 1 mg/kg, 5 mg/kg, 25 mg/kg). The general conditionof rats was recorded, and lung pathology, lung wet/dry weight ratio, the platelet count, serum vWF and ADAMTS13 were determined.Results The pathological changes of rats in the sepsis group were obvious, and the pathological features of the calcitriol group were better than those of the sepsis group, and the pathological changes of the lung tissues of the calcitriol groups were significantly alleviated compared with the sepsis group.The platelet count in the sepsis group and the calcitriol groups(1 mg/kg, 5 mg/kg)was significantly lower than that in the normal control group, while the platelet count in the calcitriol group(25 mg/kg) was not significantly different than that in the normal <br> control group, but it was still significantly lower than that in the sepsis group.The serum levels of vWF in the sepsis group were significantly higher than those in the normal group, and the serum vWF in calcitriol groups was significantly higher than that in the normal group, but lower than that in the sepsis group.The serum levels of ADAMTS13 in the sepsis group and the calcitriol groups had no significant difference between each other, but they were decreased significantly compared with the normal control. Conclusion VD inhibits the expression of vWF and improves the endothelial cells damage, which could affect the pathogenesis of sepsis, and this effect is not achieved by improving the expression of ADAMTS13.The decrease of circulation platelets in sepsis is mainly related to the injury of endothelial cells and vWF release.
Keywords：SepsisA disintegrin-like and metalloproteinase with thrombospond in type -1 domain 13(ADAMTS13)Vitamin D(VD)Von Willebrand factor(vWF )
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1079-1082 )