Study on the mechanism of the protective effects of Paeoniflorin on the mice with acute liver injury
FEI Li -bo
DI Jia
NIE Shi -nan
Abstract：Objective To investigate the protective effects of Paeoniflorin ( PAE) on the mice with acute liver injury ( ALI ) induced by CCl 4 .Methods The ALI model was prepared by CCl 4 injected intraperitoneally .Then, the therapeutic effects of PAE on the model were evaluated by the activity determination of serum alanine aminotransferase ( ALT) and aspartate aminotransferase ( AST) and hepatic inflammation and lipid metabolism disorder , and the changes of hepatic pathohistology following the PAE treatment .Results Following the treatment , PAE reduced the elevated ALT and AST activities and interleukin -1β( IL -1β), IL -6, tumor necrosis factor -α( TNF -α), triglyceride ( TG) , total cholesterol ( TC) and very low density lipoprotein ( VLDL) content induced by CCl4 significantly, and downregulated the sphingosine -1-phosphate levels in liver homogenates , and inhibited hepatic sphingosine kinase 1 ( SphK1 ) and nucleoprotein -nuclear transcription factor -kappaB (NF-κB) protein expression.Furthermore, the pathological changes were also significantly improved in the mice of PAE treating group .Conclusion The protective effects of PAE on the mice with ALI induced by CCl4 may be related to its inhibition of SphK1/S1P signal pathway.
Keywords：Paeoniflorin(PAE)Acute liver injurySphK1/S1P signal pathwayCCl4
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1032-1035 )