Effect of marrow derived mesenchymal stem cells on the treatment for acute pulmonary injury in sepsis mice

doi:10.3969/j.issn.1002-1949.2015.11.003

Abstract：Objective To explore the possible mechanism and protective effect of marrow derived mesenchymal stem cells (BMSCs) on sepsis mice with acute lung injury (ALI).Methods Sepsis mice with ALI were induced by cecal ligation and puncture (CLP).One hundred and twenty -five Balb/c mice were randomly divided into five groups , twenty -five mice for every group .Sham control group (C group), BMSCs treatment groups (L, M, H group) and ALI group (CLP group). Five minutes after CLP, the mice in BMSCs treatment groups were intravenous administrated BMSCs though caudal vein of the mice (1 ×104 for L group, 1 ×105 for M group, 1 ×106 for H group), the other two groups were given normal saline of the same quantity .Fifteen mice in each group were used to observe the survival status and the others were used to collect samples for assessment of histology , lung wet to dry ratio (W/D) arterial partial pressure of oxygen and carbon dioxide (PaO2 and PaCO2), lactic acid, level of serum TNF-α, IL-10 and soluble E selectin .Results Compared with CLP group , the survival rate in H group at 24 h and 48 h were significantly improved .What's more, there was a significant decrease in W/D, lung injury score, PaCO2, level of lactic acid, serum TNF-αand souble E selectin, a significant increase in PaO2 and level of serum IL -10, in H group mice compared with CLP group mice at 24 h.Conclusion Treatment with BMSCs significantly reduces CLP -induced acute lung injury in Balb/c mice by inhibiting serum level of TNF -α, souble E selectin and increasing level of serum IL-10, and the effect is related to its dose .