The secretion function of polymorphonuclear cells after lipopolysaccharide stimulation when co-cultured with endothelial progenitor cells
Abstract：Objective To explore the influence of endothelial progenitor cells ( EPC ) on polymorphonuclear cells (PMN) secretory factors stimulated by lipopolysaccharide (LPS).Methods EPC and PMN were separated and cultured respectively , and they were divided into three groups .EPC were incubated in a 24-well plate at a density of 1 ×105 per well.After adherence, PMN with the same density were added in the medium , and 1μg/mL LPS was applied to the mixture for 6 hours.There are same density PMN in PBS and LPS groups stimulated by equidensity PBS and LPS respectively . Supernatant and cells were collected for determination , and the changes of tumor necrosis factor -α(TNF-α), interleukin-1β(IL-1β), neutrophil elastase (NE) and matrix metalloproteinases -9 (MMP-9) in three groups were detected .Results TNF-α, IL-1β, NE and MMP-9 contents were remarkably increased with stimulated by LPS compared with PBS group (P<0.05).When EPC were added, all productions significantly reduced ( P <0.05 ).Conclusion EPC can inhabit the activation of PMN after stimulating with LPS .
Keywords：Endothelial progenitor cells (EPC)Polymorphonuclear cells (PMN)Tumor necrosis factor -α(TNF-α)Interleukin-1β(IL-1β)Neutrophil elastase (NE)Matrix metalloproteinases -9 (MMP-9)
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 859-862 )