Expression and significance of TLR2 in podocyte of mice with the acute kidney injury
Abstract：Objective To investigate the expression of Toll -like receptor 2 ( TLR2 ) in the lipopolysaccharide ( LPS ) -induced septic acute kidney injury ( AKI ) mice and the potential mechanism.Methods Using LPS-treated mice as a model of septic AKI , forty mice were randomly divided into normal control group (n=20), LPS group (n=20).Urinary albumin levels of 24 hours, blood urea nitrogen and creatinine were tested .The expression and distribution of TLR 2 in kidney were determined by immunohistochemistry .Reverse transcriptional -polymerase chain reaction ( RT-PCR ) was performed to evaluate the mRNA levels of TLR 2.Results In the LPS -treated mice, urinary albumin levels increased associated with the elevation of blood urea nitrogen and creatinine levels , indicating the successful induction of septic AKI .Compared with the control group , the expression of TLR2 in podocytes and glomerular endothelial cells of kidney significantly increased in LPS group .TLR2 mRNA expression was up -regulated in LPS group compared with the control group (5.52 ±0.21 vs 1.00 ±0.05, P <0.01).Conclusion LPS treatment led to an significant increase of TLR2 expression , especially in the podocytes and glomerular endothelial cells of kidney .The activation of TLR2 signal way might play an important role in the pathogenesis of septic AKI .
Keywords：Toll like receptor 2 (TLR2)lipopolysaccharide (LPS)SepsisAcute kidney injury(AKI)Podocyte
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 798-801 )