Effect of insulin glargine on heart and kidney damage in burned rats with delayed fluid resuscitation

doi:10.3969/j.issn.1002-1949.2015.08.017

Abstract：Objective To investigate the protective effect of insulin glargine against heart and kidney damage in burned rats with delayed fluid resuscitation.Methods Twenty-four male Sprague-Dawley ( SD) rats were randomly divided into three groups with 8 rats in each group:sham burn group, burn control group and burn plus insulin group.The sham burn group was immersed into 37 ℃ warm water for 15 seconds to simulate the burn process.The burn control group and burn plus insulin group were immersed into (95 ±0.5)℃ hot water for 15 seconds to make a rat model of 30% total burn surface area ( TBSA) ,Ⅲ degree burn injury rats received an intraperitoneal injection of physiological saline (40 mL/kg) at 6 h after burn.Insulin glargine [1.0 U/(kg· d)] was administered subcutaneously at 2 h postburn in burn plus insulingroup, and subcutaneous injection of the same volume physiological saline in the burn control group.Rats were sacrificed 24 h after burn, abdominal aorta blood was gathered and blood glucose, lactate dehydrogenase ( LDH ), α-hydroxybutyrate dehydrogenase (α-HBDH) , creatine kinase ( CK) , blood urea nitrogen( BUN) , creatinine ( Cr) were analysised.Oxidation and antioxidation parameters in heart and kidney obtained from rats, such as malondialdehyde (MDA), xanthine oxidase (XO), myeloperoxidase (MPO), superoxide dismutase 1 (SOD1), catalase (CAT), glutathion peroxidase (GPx) and the total antioxidant capacity (T-AOC), these parameters were detected by spectrophotometry.Creatine kinase MB ( CK-MB) was determined by immunosuppression.Results (1) Compared with the sham burn group, LDH, α-HBDH, CK, CK-MB, BUN, Cr were significantly higher in the burn control group (P<0.05).In the burn plus insulin group, LDH,α-HBDH, CK, CK-MB, BUN, Cr were significantly lower in comparison with the burn control group (P<0.05).(2) Compared with the sham burn group, the burn control group MDA, XO, MPO in the heart and kidney tissues were significantly higher (P<0.05); SOD1, CAT, GPx, T-AOC were significantly lower (P<0.05).Compared with the burn control group, the burn plus insulin group MDA, XO, MPO in the heart and kidney tissues were significantly lower ( P<0.05);SOD1, CAT, GPx, T-AOC were significantly higher (P<0.05).(3) Compared with the sham burn group, the blood glucose in burn control group and burn plus insulin group were significantly higher, the blood glucose in the burn plus insulin group was significantly lower in contrast to the burn control group (P<0.05).Conclusion Insulin glargine possesses the protective effect against heart and kidney damage in burned rats with delayed fluid resuscitation, which may be associated with early glycemic control and alleviation of tissues lipid peroxidative injury.