The effect of necrostatin-1 on the expression of hepatic MIP-1αin hemorrhagic-traumatic shock rats

doi:10.3969/j.issn.1002-1949.2015.08.016

Abstract：Objective To investigate the effect and mechanism of necrostatin-1 (Nec-1) on the expression of hepatic macrophage inflammatory protein-1α( MIP-1α) in hemorrhagic-traumatic shock rats.Methods The model was Sprague-Dawley ( SD) rats suffered hemorrhagic -traumatic shock.A number of 40 male SD rats were divided into model group, DMSO group, Nec-1 group and sham group with 10 rats in each group by randomized digital number method.Rats in sham group were only received anesthesia, separating and ligating blood vessels, without trauma induced hemorrhagic and reperfusion. Rats in model group were received hemorrhagic -traumatic shock without other intervention.Rats in Nec-1 group were received 1mg/kg Nec-1 through femoral vein 5 minutes before reperfusion, while the rats in DMSO group were received the same amount of solvent.The serum and liver tissues of each group were collected 24 hours after reperfusion.Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected by automatic biochemistry analyzer.The pathology changes in liver were observed by hematoxylin -eosin ( HE ) staining.The expression of MIP-1αin serum was detected by ELISA.The MIP-1αmRNA in the liver was determined by reverse transcription-polymerase chain reaction ( RT -PCR ) .The protein expressions of MIP -1αwere assessed by Western blotting analysis.Results Compared with model group and DMSO group, there was no different between the expressions of ALT, ATS and MIP-1αin serum (P>0.05).Levels of serum ALT, AST and MIP-1α24 hours after reperfusion in Nec-1 group were significantly decreased compared with model group and DMSO group (P<0.05).Under light microscopy, it was noted that hepatic lobule destroyed, the blood extravasated, and the immunocyte infiltrated.Compared with DMSO group and model group, the expression levels of MIP-1αprotein and MIP-1αmRNA in Nec-1 group were significantly decreased (P<0.05).Conclusion Nec-1 can remarkable protect the liver of rats with hemorrhagic-traumatic shock, decrease the expression of MIP-1αprotein, reduce the infiltration of immunocyte.