Effect of mild hypothermia on the coagulation with traumatic haemorrhagic shock in rabbits

PENG Luo-gen; WU Ruo; ZHAO Hui-min

doi:10.3969/j.issn.1002-1949.2015.08.013

Abstract：Objective To investigated the mild hypothermia effect on coagulation in rabbits subjected to traumatic and hymorrhagic shock.Methods Twenty rabbits were randomly divided into two groups with ten animals in each group: sham control and mild hypothermia ( group T/H ) , traumatic hymorrhagic shock and mild hypothermia ( group T/HS).Hypothermia was induced by anesthesia induction, with a cold blanket and ice bag.Hemorrhage was induced by bleeding 35% of total blood volume.Capacity for coagulation was measured by thromboelastometry ( TEG ) and then the routine blood, temperature ( T ) , heart rate ( HR ) , shock index ( SI ) , mean arterial pressure ( MAP ) , hemoglobin ( HGB) , respiration ( R) and related physiological indexes were recorded at different time including before shock ( t0 ) , 30 minutes after shock ( t1 ) , 2 hours after hypothermia ( t2 ) , 2 hours after rewarm ( t3 ) .Results ①Baseline parameters were not significantly different among the groups.The survival rates in both groups were 100%.Compared with group T/H, the level of MAP and HGB in group T/HS were significantly lower at t1, t2 and t3 timepoints (all P<0.01);while R, HR and SI in group T/HS were significantly higher at t1 timepoint ( all P<0.01) and SI were significantly higher at t2 timepoint (P<0.01).The level of MAP, HR, R and SI of control group didn't change significantly in all of the time points.②In group T/HS, the initial clotting time (r), the maximum amplitude (MA) was reduced compared with group T/H from t1 to t3(all P<0.05).The clotformation time (K) was not significantly different at t1 and t2 timepoints (all P>0.05).The rate of clot formation (Angle) and the coagulationindex ( CI) in the two groups at t1 -t3 timepoints did not change significantly compared with baseline level (all P>0.05).③PLTs in group T/HS were reduced in t1, t2 and t3 timepoints (all P<0.05) and PLTs in t2 and t3 timepoints were significantly lower than that in t1 timepoint (all P<0.05). Conclusion Based on the results of the present study we suggest that mild hypothermia and rewarm can reduce the PLTs, then mild hypothermia can be safely performed after stabilization following the earliest traumatic haemorrhagic shock in rabbits.Mild hypothermia does not significantly effects on the holistic coagulation system in our model.