Effect of C5a anti-sense peptide on myocardial neutrophil infiltration and the expression of P-selectin in septic mice

doi:10.3969/j.issn.1002-1949.2015.08.004

Abstract：Objective To investigate the effects of C5a anti -sense peptide on myocardial neutrophil infiltration in septic mice.Methods Cecal ligation and puncture ( CLP ) was used to establish the sepsis model.Mice were randomly divided into four groups:control group, sham operation group, sepsis group and C5a anti-sense peptide treatment group.Pathological changes of myocardial tissue were observed at intervals of 2 h, 4 h, 8 h and 12 hours after CLP. The activity of myeloperoxidase ( MPO) in myocardium was detected by kit.Myocardial P-selectin mRNA expression was respectively determined by real-time PCR ( RT-PCR) in each group.Western blotting and image analysis software were employed to semi-quantitatively calculate the dynamic changes of P-selectin protein expression level. Results The microscopic examination showed loose alignment with vacuolization, interstitial swelling in myocardial tissue of mice in model group 4 hours after CLP, and those changes significantly lessened in mice of the treatment group.MPO activity in myocardium were significant higher in sepsis group and treatment group than the other groups.Compared to control and sham operation group, the mRNA and protein expression level of P-selectin was significant higher in the other groups.Myocardial P-selectin mRNA level in treatment group was lower than in sepsis group ( P <0.05 ) .However, myocardial P -selectin protein expression level was greatly decreased in treatment group (P<0.05) while compared with sepsis group, but was still higher than those in control group and sham operation group (P <0.05).Conclusion C5a anti -sense peptide reduces the expression level of P-selectin of myocardial in septic mice.