The effects of hydroge n sulfide on the electrolytes and renal function in sepsis rast

doi:10.3969/j.issn.1002-1949.2015.08.003

Abstract：Objective To investigate the effects of hydrogen sulfide ( H2S ) on serum electrolytes and renal function in sepsis rats .Mtehods A total of 92 male Spargue-Dawley rats were used in the present study.Twenty rats were randomly divided into 5 groups: normal rats group, cecal ligation puncture( CLP) 6 h, 12 h, 24 h group ( n=5/group) .Seventy-two rats randomly divided into sham operate group, CLP+saline group (n=18), CLP+sodium hydrosulfide (NaHS) group (n=18) and CLP +propargylglycine ( PAG ) group ( n=18 ) .Rats were administrated with saline, NaHS or PAG respectively by peritoneal injection 1 h before operation.Rats were sacrificed at designated time and serum was collected to detect serum electrolytes, creatinine ( Cr ) and urea nitrogen ( BUN ) concentrations.Results Compared with normal rats, serum Na+level increased followed by a decline, then returned to normal level after CLP.Serum Cl-level increased after CLP till 6 h.After 6 h, serum Cl-level started to decline in a time-dependent manner.Serum K+, Cr and BUN levels increased in a time-dependent manner after CLP.Serum Ca2+level decreased at all time points after CLP.Compared with CLP+saline group, serum Na+, Cl-, K+, Cr and BUN levels were significantly decreased in CLP+NaHS group.There was no effect on serum Ca2+level at all time points.At all time points in CLP+PAG group, serum Na+, Cl-, K+, Cr and BUN levels were significantly elevated and serum Ca2+level do not significantly changed.Conclusion Sepsis induce serum electrolytes disturbance. Renal function is injured at early stage of sepsis and deteriorates in a time-dependent manner.H2 S regulates electrolyte levels and renal function during sepsis and the effects on kidney may involve in regulation of serum electrolyte concentrations during sepsis.