Role of different cell architectural in HBO therapy of delayed encephalopathy
Abstract：Objective To investigate the effects and mechanism on neuronal apoptosis of cortex different cell architectural after acute carbon monoxide ( CO) poisoning and hyperbaric oxygen ( HBO) therapy.Methods The animal model of acute CO poisoning was established .Then the SD rats were treated with hyperbaric oxygen therapy .Immunohistochemistry and immunofluorescence were used to determine the expression level of Caspase -3, NeuN and MMP-9 at 1 d, 3 d, 7 d, 14 d and 21 d. Results Different cell of rat cortex involved in neuronal apoptosis after acute CO poisoning and HBO therapy;the internal-granular layer and internal -pyramidal layer of cortex most sensitivity in apoptosis after acute CO poisoning .Caspase-3 and MMP-9 regulates neuronal apoptosis:it began to increase at 1 d, reached the maximum at 3 d, started to decrease at 7 d;for the CO poisoning rats treated with HBO therapy, MMP-9 and Caspase-3 in cortex were obviously decreased , especially in internal -granular layer and internal-pyramidal layer .On 7 day, the inhibitory effect of HBO therapy is the most obvious . Conclusion Neuronal apoptosis of cortex depend on different cell architectural , internal -granular layer and internal -pyramidal layer are most sensitive in the 6 layers.MMP -9 expression may responsible on the apoptosis and may related with matrix degradation .Also, the expression of Caspase -3 and MMP-9 may related with the function of neurons .HBO therapy may control the expression of apoptosis related proteins and reduce the expression of MMP -9 and Caspase -3, then inhibit neuronal apoptosis.
Keywords：Hyperbaric oxygen ( HBO) therapyCarbon monoxide poisoningMatrix metalloproteinase -9 ( MMP-9)Neuronal apoptosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 651-655,656 )