The influence of oxygen concentration on brain function and brain mitochondrial energy metabolism after cardiopulmonary resuscitation in rats
WANG Lian-fu
WANG XU-dong
Abstract：Objective To investigate the influence of difference oxygen concentration on brain function and brain mitochondrial energy metabolism after cardiopulmonary resuscitation in rats .Methods Forty rats were randomly divided into 5 groups:sham group, 100%O2 CA/CPR group, 75%O2 CA/CPR group, 50%O2 CA/CPR group and 21%O2 CA/CPR group, 8 rats in each group.The sham group only took tracheal intubation , without cardiac arrest .The other groups anesthesia , connect cardiac monitor , tracheal intubation and connect the animal breathing machine in turn .Occlusion the tracheal at end-expiratory , make asphyxia into cardiac arrest , open the breathing machine , inhale different oxygen concentration, chest compressions and adrenaline injection to make spontaneous circulation return , stop CPR.All rats were conducted nerve function evaluation by the score of nerve behavior of NDS .ATP of cerebral tissue was measured by high performance liquid chromatography method .Spectrophotometry was used to detect the mitochondrial respiratory chain complexes Ⅲ state, Ⅳ state activity.Results Compared with the sham group , the nerve function scores and brain ATP content in 21%O2 CA/CPR group and 100%O2 CA/CPR group were remarkably reduced (P<0.05), and the State 3 respiration rate significantly decreased (P<0.05), State 4 respiration rate significantly accelerated (P<0.05). Conclusion In the course of CPR , neither low oxygen concentration and high concentration has beneficial on brain function and brain mitochondrial energy metabolism after cardiopulmonary resuscitation.
Keywords：Cardio-pulmonary resuscitationOxygen concentrationNerve function deficitMitochondrial energy metabolism
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 642-645 )