The expression and significance of IL -6/STAT3 signaling pathway on the hypothalamus -pituitary-adrenal axis in the early stage of sepsis rats
WANG Xiao-dan
NIE Wen-long
ZHANG Yu-xiang
Abstract：Objective To explore the inner link between interleukin -6 ( IL -6 )/signal transducer and activator of transcription 3 ( STAT3 ) signaling pathway and hypothalamus -pituitary -adrenal axis ( HPAA) excessive activation in the early stage of sepsis rats .Methods Twenty -four adult male Wistar rats were randomly divided into Control group ( n=8 ) , sham-operated group ( n=8) and CLP group (n=8).The septic rat models were challenged by CLP , executed after 6 hours, the hypothalamus, pituitary and adrenal gland was isolated .Expression levels of CRH, IL-6, STAT3 and SOCS3 mRNA in hypothalamus , POMC, IL-6, STAT3 and SOCS3 mRNA in pituitary and IL -6, STAT3 and SOCS3 mRNA in adrenal gland were quantified by real -time quantitative PCR ( RT -PCR).Results Compared to Control group and Sham group: CRH, IL -6, STAT3 and SOCS3 mRNA expression levels were up -regulated significantly (P<0.01) in the hypothalamus tissues of CLP group;POMC, IL-6, STAT3 and SOCS3 mRNA expression levels were up -regulated significantly (P<0.01) in the pituitary tissues of CLP group; IL-6, STAT3 and SOCS3 mRNA expression levels were up-regulated significantly (P<0.01) in adrenal gland tissues of CLP group .Expression levels of all purpose gene were not different in Control group and Sham group .Conclusion IL -6/STAT3 signaling pathway has a close relationship with HPAA excessive activation in the early stage of sepsis rats.IL-6/STAT3 signaling pathway may be regarded as a novel therapeutic target for HPAA excessive activation of the sepsis .
Keywords：SepsisHypothalamus-pituitary-adrenal axis(HPAA)Interleukin-6(IL-6)Signal transducer and activator of transcription 3( STAT3)Real-time quantitative PCR( RT-PCR)
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 585-588 )