Effect of alcohol combined with hypothermia on cerebral resuscitation after cardiac arrest in rabbits

doi:10.3969/j.issn.1002-1949.2015.06.018

Abstract：Objective To investigate the protect affect of ethanol with hypothermia for cardiopulmonary resuscitation ( CPR ) of rabbit brain.Methods Ventricular fibrillation ( VF ) was induced by the external electricity, eighty adult rats were randomly divided into five groups ( n=16 ):sham-operated group (group A), control group (group B), hypothermia group (group C), alcohol group ( group D ) , alcohol combined with hypothermia group ( group E ) . Group A rabbits were endotracheal intubation, anesthesia and surgery at room temperature, Group B cause cardiac arrest and parallel conventional recovery.Group C give local mild hypothermia after ROSC.Group D were given 1.5 g/kg ethanol ( Diluted with 0.9% saline to 10 mL, pumped through the internal jugular vein by micro-pump in 15 min), Group E were given 1.5 g/kg ethanol (Diluted with 0.9%saline to 10 mL, pumped through the internal jugular vein by micro-pump in 15 min) with local mild hypothermia after ROSC.Determine the serum content of protein S100βcontent of each group before the trial and 12 h after the trial.Twelve hours after the trial, the rabbits were sacrificed to determine the brain water content ,brain dead volume .Results ①The serum S100βcontent:compared with group A, group B、C、D、E showed a significantly higher serum S100βcontent, especially group B (P<0.05);and group E showed the lowest serum S100βcontent among group B、C、D、E ( P <0.05 ); ②The brain water content, brain dead volume:compared with group A, group B、C、D、E showed significantly higher brain water content and brain dead volume, especially group B (P<0.05); and group E showed the lowest brain water content, brain dead volume among group B、C、D、E (P<0.05).Conclusion Ethanol combined with hypothermia show synergic effects on cerebral resuscitation after cardiac arrest.