Study of endocan and inflammatory factors expression during lipopolysaccharide-induced lung injury in mice

LI Yan; SIMA Xin-yuan; WU Yang

doi:10.3969/j.issn.1002-1949.2015.06.016

Abstract：Objective To explore endocan and inflammatory factors expression during lipopolysaccharide ( LPS) induced acute lung injury ( ALI ) in mice, and to explore their roles during ALI in mice.Methods LPS-induced mice ALI models were assigned into control groups and LPS groups (0, 6, 12, and 24 h according to time points pre-scheduled).The ALI models were injected with 5 mg/kg LPS through tail vein, and the normal control groups were administrated with equal volume phosphate buffer solution (PBS). Mice were anesthetized, and blood samples were collected.Mice were then killed; lungs were obtained to perform next experiments.Lung samples were processed and hemotoxylin and eosin staining ( HE ) were done to examine the histopathological changes. Myeloperoxidase ( MPO) and wet to dry of lungs were measured.Levels in mice serum of tumor necrosis factorα( TNF-α) , interlukin -6 ( IL -6 ) , high mobility group box1 ( HMGB1 ) were analyzed. Expression of nuclear factor-κB ( NF-κB) and phosphorylated nuclear factor-κB were tested by immunoblotting assays.Results Compared with the control groups, LPS administration resulted in severe lung injury, increased lung wet/dry ratio.MPO levels, expression of TNF -α, IL -6, and HMGB1were elevated significantly after LPS treatment.Meantime, LPS also increased endocan and phosphorylated NF -κB expression in mice.Conclusion The expression changes of inflammatory factors and endocan may suggest the development process during LPS induced lung injury.The present study may offer beneficial reference for treating ALI, and decreasing multiple organs failure ( MOF) in earlier phase clinically.