Effect of Xuebijing combined with ischemic preconditioning on liver ischemic reperfusion in the jury in rats
Abstract：Objective To investigate the effect of Xuebijing combined with ischemic preconditioning on liver ischemic-reperfusion injury in rats.Methods One hundred and eighty male SD rats were randomly divided into five groups: sham -operation group ( group S ) , ischemia -reperfusion group ( group IR) , Xuebijing preconditioning group ( group XBJ) , ischemic preconditioning group (group IP) and Xuebijing combined with ischemic preconditioning group (group XBJ+IP) and each group was randomly divided into three time points (6, 12, 24 h).Ischemia-reperfusion model were established according to Pringle's method.After 30 min ischemia and 6, 12, 24 h reperfusion of the liver, the concentrations of ALT and AST in serum were measured, the liver histological changes, cell apoptosis and expression of heme oxygenase-1 were observed under optical microscope.Results At the same time point, serum concentrations of ALT and AST in group IR, XBJ, IP and XBJ+IP were higher than those in group S (P<0.01 or P<0.05, except the AST of group XBJ+IP at 24 h);group XBJ and IP were significantly lower than those in group IR ( P <0.01 ); group XBJ +IP were significantly lower than those in group XBJ and IP ( P<0.01); the serum concentrations of AST in group XBJ was significantly lower than those in group IP (P<0.01).Pathological score in IR, XBJ, IP and XBJ+IP group was higher than that in group S ( P<0.01, except the XBJ+IPC group at 24 h);group XBJ and IP was significantly lower than that in group IR ( P <0.01 ); group XBJ +IP was significantly lower than that in group XBJ and IP (P<0.01).The apoptotic index in group IR, XBJ, IP and XBJ+IP was significantly higher than that in group S;group XBJ and IP was significantly lower than that in group IR (P<0.01), group XBJ +IP was significantly lower than that in group XBJ and IP (P<0.01).The HO-1 score in group IR was significantly higher than that in group S (P<0.01);group XBJ and IP was significantly higher than that in group IR ( P <0.01 ); group XBJ +IP was significantly higher than that in group XBJ and IP (P<0.01).Conclusion Both of Xuebijing and ischemic preconditioning can protect liver from ischemia reperfusion injury in rats to some extent, and the combined application gives better effects.
Keywords：XuebijingIschemic preconditioningLiverIschemia-reperfusion injury
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 544-548 )