Mechanism of antibiotic resistance of Pseudomonas aeruginosa infection in ICU patients and an evaluation about the corresponding phenotypic methods
Abstract：Objective To detect Metallo-beta-lactamase ( MBL) producing aeruginosa( PA) isolates from ICU patients, to analysis of mechanism of antibiotic resistance of PA to compare and evaluate different phenotypic methods currently in use and to determine antibiograms.Methods A prospective study was undertaken to detect MBL in PA isolates obtained from 520 ICU samples.A total of 147 strains were recovered from patients admitted in ICU, and screened for imipenem resistance by automatic bacteria identification system.Detection of MBL was further done by imipenem-EDTA disk synergy test and combined disk test.Results Out of 147 isolates, 33 isolates (22.4%) were imipenem resistant.All 33 imipenem resistant PA strains, when further tested, were positive for MBL production by combined disk test, but only 24 showed positive results by imipenem -EDTA disk synergy test. Conclusion MBL production was the main resistance mechanism in the 33 carbapenem resistant PA isolates collected, with multidrug resistance associating significantly with MBL production in PA from ICU.
Keywords：Metallo-beta-lactamase( MBL)Pseudomonas aeruginosa( PA)Antibiotic resistance
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 510-513 )