The expr ession of highm obility group box 1(HMGB1) and protective fe fects of ethylp yruvate on intestinal lesion during sepis s in rats

doi:10.3969/j.issn.1002-1949.2015.06.001

Abstract：Objective To investigate the role of high mobility group box 1 ( HMGB1 ) in intestinal tract injury of sepsis in rats and the mechanism of its treatment with ethyl pyruvate.Methods A rat sepsis model was established by cecal ligation and puncture ( CLP ) .Sixty SD rats were randomly divided into the sham group (Group S), sepsis group (Group C)and ethyl pyruvate treated group ( Group M) with 20 rats in each group.Group M received intraperitoneal injection of ethyl pyruvate ( 40 mg/kg ) immediately after operation, while Group S and Group C were both given intraperitoneal injection of Lactated Ringer's Solution.Five rats in each group were picked up at 3, 6, 12, 24 h after CLP to have intestinal tract pathological damage observed by microscope, and the plasma levels of diamine oxidase ( DAO) and D-lactic acid measured, and the HMGB1 expression of small intestinal tissues detected by immunohistochemical method and reverse transcription -polymerase reaction method ( RT-PCR) .Results There was no significant difference at each time point of each detection index in Group S.In Group C and Group M, each detection index was rising with increased postoperative time.The intestinal histological scores of Group C, the HMGB1 expression of small intestinal tissues of Group C and M were higher than that of Group S at 6, 12, 24 h,while in Group M , the scores were higher than that of Group S at 12, 24 h (P<0.05).The plasma levels of diamine oxidase ( DAO) and D-lactic acid of Group C and M were higher than those of Group S at each time point.Compared with Group C, the intestinal histological scores of Group M were lower at 24 h, and the expression level of HMGB1 mRNA and the plasma levels of DAO and D-lactic acid were lower at 6, 12, 24 h, and the expression of HMGB1 protein in small intestinal tissue was lower at 12, 24 h ( P<0.05).Conclusion The degree of intestinal tract injury was associated with the HMGB1 expression of intestinal tissues.Ethyl pyruvate could reduce sepsis-induced intestinal tract injury, which was likely due to the inhibition of HMGB1 expression in intestinal tissues.