The protective effect of ulinastatin on myocardium in rats subjected to 50% total body surface area burn injury

doi:10.3969/j.issn.1002-1949.2015.05.017

Abstract：Objective To investigate the protective effects of ulinastatin on myocardium in rats following 50%total body surface area burn injury and its relationship with the expression of protective protein.Methods Forty male Sprague -Dawley rats were randomly divided into scald group and ulinastatin group.Rats in the two groups were subjected to 50% total body surface area third-degree burns.Immediately after scald rats were intravenously given either 1 mL saline in scald group or 1 mL saline containing ulinastatin ( 40 000 U/kg ) in ulinastatin group.Blood of rats in each group was withdrawn from the abdominal aorta at 6 hours and 24 hours after injury for measurement of plasma creatine kinase-MB ( CK-MB) activities;the rates of tissue water content in heart were determined by the dry/wet weight.Heart tissues were harvested for the measurement of acetylated histone H3 by Western Blot and HSP70 by ELISA.Results The CK-MB and the water content of myocardial tissue after 6 h injury in ulinastatin group were (1638.3 ±339.2)U/L and (68.14 ±1.93)%, respectively, which were significantly lower than those in scald group [(2271.8 ±350.9)U/L, (83.02 ±2.12)%, respectively;P<0.05)], but the changes in measurement had no significant difference after 24 h injury between the two groups (P>0.05).The expression of histone H3 and heat shock protein 70 (HSP70) after 6 h injury in ulinastatin group were obviously higher than those in scald group ( P<0.05 or P<0.01), but the indexes had no significant difference after 24 h injury between the two groups ( P>0.05).Conclusion The results indicate that ulinastatin significantly alleviate myocardial injury and inhibit tissue edema, reduce in early stage of burn injury.These protective effects may be due to its increasing expressions of histone H3 and HSP70 may be related to improvement of the tolerance heart to burn injury.