Drug resistance analysis and distribution of carbapenems-resistant Klebsiella pneumonia

doi:10.3969/j.issn.1002-1949.2015.05.010

Abstract：Objective To evaluate the susceptibility of carbapenems-resistant Klebsiella pneumoniae ( CRKP ) to antimicrobial agents, and to explore the distribution of clinical isolates of CRKP.Methods Fifty-four strains from different clinical specimens and 13 strains from ventilator CRKP were collected. Susceptibility to commonly used antimicrobial agents were detected by disc diffusion, the MICs of tigecycline were determined using E-test strip.Double disk synergy test was employed to examine ESBLs.Results Of the 54 clinical isolates, sputum was the most common source of CRKP ( n =39 ) , then blood and urine ( n =4 ) .The most common clinical department was the Intensive Care Units (ICU, 44.44%), then Digestive System Department (22.22%), respiratory department (9.26%) .According to the antimicrobial susceptibility test, all the isolates showed the high resistance rate to multiple antibiotics over 90%, of which, the resistance rates of ceftazdime, ceftriaxone, piperacillin up to 100%.The lowest resistance rates were tigecycline (4.48%), polymyxin B (23.88%), tetracycline (26.87%).The ventilator isolates showed a antibiogram parallel to clinical isolates with no statistical difference.Of the 67 isolates, 28 ( 41.79%) strains producing ESBLs. Conclusion CRKPs are widespread in clinical and mainly in ICU.The resistant rate and multi-drug resistant of CRKP is tremendous.The treatment of clinical infections due to multiresistant CRKP remains a challenge.Tigecycline has effective antimicrobial activity against CRKP.