Interventional study of Shengjiang San on Th1/Th2 and Th17/Treg imbalances in sepsis mice

doi:10.3969/j.issn.1002-1949.2015.05.005

Abstract：Objective To investigate the intervention of Chinese herb Shengjiang San ( SJS) for the imbalance of Th1/Th2 and Th17/Treg cells ratio in sepsis mice.Methods Eighty experimental mice were randomly divided into four groups: control group of 8 cases, sham operation group of 24 cases, sepsis group of 24 cases and TCM participation group ( SJS group) of 24 cases.After CLP of sepsis model, Th1, Th2, Th17 and Treg cells in the proportion of hole CD4 +Th cells and Th1/Th2, Th17/Treg ratio were compared respectively in each group before and after (6 h, 12 h and 24 h) SJS treatment.Results ①Proportion of Th1 cells was increasing in sepsis group and decreasing in SJS group at 24 hours.In sepsis group, it was higher than that in control group, sham operation group and SJS group at 12 hours and 24 hours (P<0.05 or <0.01).There was no significant difference in proportion of Th2 cells among all groups at each time point(P>0.05).Proportion of Th17 cells was increasing in sepsis group in 24 hours and it was higher in sepsis group than that of in control group, sham operation group and SJS group at 12 hours and 24 hours (P<0.01).In SJS group, it was higher than in control group, sham operation group at 12 hours and 24 hours (P<0.01).Proportion of Treg cells was increasing both in sepsis group and SJS group at 24 hours.In control group, it was lower than that in sham operation group, sepsis group and SJS group at each time point(P >0.05).In sepsis group, it was higher than that in sham operation group and SJS group at 24 hours ( P <0.05 or <0.01).②Th1/Th2 cells ratio in sepsis group was higher than that in control group, sham operation group and SJS group (P<0.05 or <0.01) while there was no significant difference of Th1/Th2 cells ratio between SJS group and control group(P>0.05)at each time point.In sepsis group, it was higher than in sham operation group and SJS group at 24 hours (P<0.05).Th17/Treg cells ratio in sepsis group was higher than that in control group and sham operation group at each time point ( P<0.05 or 0.01) while it was higher than that in SJS group at 6 hours (P<0.01).In SJS group, it was higher than that in sham operation group and control group at 12 hours and 24 hours (P<0.01).Conclusion Chinese herb Shengjiang San has an effective benefit to the imbalance of Th1/Th2 and Th17/Treg in sepsis mice.