A sepsis-inducedacute kidney injury mouse model and its kidney injury evaluation
Abstract：Objective To examine the mortality and acute kidney injury in the cecum ligation and puncture ( CLP ) model with different ligation proportion.Methods Eighty -one mice were assigned to 3 different experiments.①Survival analysis between 25% vs 50% ligation of CLP model.②Analysis of 3 renal injury biomarkers (serum creatinine, NGAL, cystatin C) and cytokine of IL-6.③Renal histopathology at the most severely injured time point.Results The mortality rate of 50%ligation of CLP model at 48 hours was 100% whereas the 25% ligation mortality at 24 hours was 7%, and the 48 hours mortality was about 50%.Renal biomarkers of Creatinine, NGAL increased from 2 hours and reach their peak at 24 hours, by contrast, Cystatin C and IL-6 reach the highest level at 18 and 6 hours.Apical vacuolization can be found in the histopathology of kidneys at 24 hours after CLP but with absence of necrosis.Conclusion Cecum ligation ( 25%) and puncture through and through (2 holes) can mimic sepsis induced AKI, study of biomarkers of renal injury show kidney injury may most severely at 24 hours.
Keywords：Acute kidney injury( AKI)SepsisCecum ligation and punctureBiomarkersCytokine
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 394-397 )