Protective effect of volume resuscitation on the ability of spatial learning and memory in a rat model of acute massive hemorrhage

WU Jian-nan; LIU Xiu-zhen; LV Yi-dong

doi:10.3969/j.issn.1002-1949.2015.04.020

Abstract：Objective The present study aims to investigate the protective effect of volume resuscitation fluids on the ability of spatial learning and memory in a rat model of acute massive hemorrhage by observing the praxiology of the rats of acute massive hemorrhage of volume resuscitation with crystalloid (lactate Ringer's solution) or colloid (hydroxyethyl starch 130/0.4 kDa).Methods Sixty male SD rats were randomly divided into 4 groups, including control group ( A, n =6 ) , acute massive hemorrhage group (B,n=18), lactate Ringer’s solution group (C,n=18), 6% hydroxyethyl starch (130/0.4 kDa) (D,n=18).All rats were navigation training in the Morris water maze for 5 days and withdrawing blood after they has learned and remembered the position of platform.Control group without treatment, the other three groups were withdrawing blood from the femoral artery, bleeding volume is about 30%of the total blood volume (65 mL/kg) .One hours later, C group and D group’ s animals were resuscitated respectively with hydroxyethyl starch( HES, 130/0.4 kDa ) and lactate Ringer’ s solution.The volume of resuscitation fluids respectively were 1 times and 3 times amount of blood withdrawn.Reversal water maze test was done on the 1 day ( T1 ) , 3 days ( T3 ) , and 7 days ( T7) after operation.After test, the rats were sacrificed immediately, stripping of rat brain tissue, determination of water content in brain tissue by dry wet method.Results In the reversal water maze test, compared with the time for find platform of A group [(14.53 ±3.37)s], B, C, D three groups [respectively:(53.32 ±10.29) s, (53.32 ±10.29) s; (42.06 ±11.47) s, (40.74 ±16.62) s;(29.26 ±7.32)s, (32.00 ±17.79)s] were significantly prolonged (P<0.05) at T1 and T3.But at T7, there were no differences between the different resuscitation groups.Compared with B group, the time of C and D groups was significantly reduced (P<0.05) at T1 and T3.Compared C group and D group, the time of D group was less than that of C group (P<0.05).At T1 and T3, the rats′brain tissue water content [respectively: (81.17 ±11.69)%, (81.00 ±6.32)%; (79.50 ±8.37)%, (79.83 ±7.53)%] of C group and D group were higher than that of A group [(77.00 ±6.32)%, P<0.05], the water content of brain tissue of D group was lower than C group (P<0.05).Conclusion Colloid (hydroxyethyl starch 130/0.4 kDa) recovery can obviously reduce the degree of brain edema and better improve the ability of learning and memory than crystalloid ( lactate Ringer's solution) group, so it has better protective effect on brain.