Effects of Xuebijing injection on cardiac function in septic rats

doi:10.3969/j.issn.1002-1949.2015.04.04

Abstract：Objective To investigate the effects of Xuebijing injection on cardiac function in cecal ligation puncture (CLP ) rats.Methods Clean healthy male Wistar rats were randomly divided into normal control group, sham operation group, CLP group and Xuebijing (XBJ) treated group.The three latter groups were divided into four groups of 12, 24, 48 and 72-hour subgroups with 6 rats in each one.Left ventricular ejection fraction ( LVEF ) of the rats were detected by echocardiography at various time points. The blood samples of each group were collected at various time points from abdominaly aorta.Serum levels of brain natriuretic peptide ( BNP ) and tumor necrosis factor -α( TNF-α) were detected by euzymelinked immunosorbent assay ( ELISA) .The sample of the cardiac muscle were harvested aseptically.The histopathologic changes of myocardial tissue were observed. Results Compared with normal control and sham operation group LVEF decreased (P<0.05); BNP markedly increased (P<0.01), the myocardial histopathologic score was increased (P<0.05) and TNF-αmarkedly increased ( P<0.01) in CLP group,and those levels were markedly improved in the Xuebijing-treated group (P<0.05或P<0.01).Conclusion Xuebijing injection could decrease the CLP rats TNF -αand BNP, and obviously ameliorate the myocardial histopathologic score and the cardiac dysfunction.