Expression and relationship of PPARγand NF -κB in peripheral blood mononuclear cells of multiple organ dysfunction syndrome patients

XU Fei; QIAO Wan-hai; WANG Li-ming

doi:10.3969/j.issn.1002-1949.2015.03.010

Abstract：Objective To study the expression and relationship of nuclear factor -kappa B ( NF-κB) and peroxisome proliferators activated receptorγ( PPARγ) in peripheral blood monouclear cells ( PBMCs) of multiple organ dysfunction syndrome ( MODS) patients.Methods In the emergency department and intensive care unit ( ICU) of our hospital, 60 MODS patients were divided into three groups with 20 cases in each group.They were MODS1 group ( APACHEⅡscore 0~10 points) , MODS 2 groups (APACHEⅡ score 11 ~20 points), MODS 3 groups (APACHEⅡ score>20 points) and control groups of 20 healthy subjects.Venous blood were sampled 1, 3, 6 d after diagnosis of MODS. Reverse transcriptase -polymerase chain reaction ( RT -PCR ) was used to detect the expression of PPARγ, NF-κB in PBMCs.The expression of PPARγ, NF-κB in the same period were compared between the MODS groups and the healthy control group.Results ①The expression of NF-κBp65 and PPARγwas low in vehicle control group.In MODS 1 group the expression of PPARγand NF-κBp65 was higher than that in vehicle control group; during the treatment period, PPARγwas significantly increased (P<0.05), but the expression of NF-κBp65 was was significantly lowered (P<0.05). PPARγexpression in MODS 2 group, MODS 3 group was lower than that in vehicle control group, and its expression was progressively decreased during the treatment (P<0.05); in contrast, expression of NF-κBp65 in MODS 2 and MODS 3 group was higher than that in vehicle control group, and its level was progressively increased during the treatment (P<0.05).The study revealed a significant negative correlation between NF -κBp65 and PPARγin MODS 1 group, MODS 2 group and MODS 3 group ( MODS 1 group:r=-0.726, P<0.01; MODS 2 group: r=-0.736, P<0.01; MODS 3 group:r=-0.721, P<0.01).There was no significant correlation in vehicle control group (r=-0.183, P>0.05).Conclusion PPARγmay protect the MODS patients by repressing the expression of NF-κB, therefore it can be used as an important indicator and potential target to determine the condition and treatment of MODS.