To correlation between aquaporin 1, 5 expression and lung injuries in acute paraquat poisoning rats
Abstract：Objective To study the relevance between expressions of aquaporin 1, 5 (AQP1, AQP5) and lung injury induced by acute paraquat poisoning in rats, and the mechanism involved. Methods Randomly divided into two groups of paraquat poisoning ( including 1, 3, 5 days three subgroups) and normal saline control group, experimental sub group of eight, control group five;rats in paraquat poisoning group were treated by intraperitoneal injection of paraquat, while those in control group were injected physiological saline instead;histopathology of animal pathology, lung wet/dry weight ratio, expression of AQP 1, 5 in rats of both groups were observed and compared.Results No difference of lung tissue of paraquat poisoning group and control group had been observed under light microscope in 1 day subgroup.Pulmonary capillary congestion, alveolar infiltration and hemorrhage, compensatory emphys ema alveolar were observed in 3 days sub-group.Alveolar epithelial hyperplasia and hypertrophy, alveolar and interstitial lung neutrophil infiltration, local abscess formation emerged in 5 days subgroup.Lung wet/dry weight ratio of control group had no difference with 1 day subgroup, but significantly lower than 3, 5 subgroups (P<0.01).Differences among 1, 3, 5 days subgroups also reached significant level (P<0.01).Quantitative of AQP1, 5 in serum and lung homogenate results showed that the highest level of it was in 3 days subgroup while 1, 3, 5 days subgroups significantly differed to each other.At the same time, difference between poisoning group and control group were significant (P<0.01).Moreover, quantity of serum AQP1, AQP1 in lung homogenate, and serum AQP5 in 1, 3 day sub groups showed a dose effect relationship with paraquat used.Conclusion AQP1, AQP5 are involved in the formation of acute lung injury in paraquat poisoning.The expression of AQP1, 5 is related with the degree of pulmonary edema.
Keywords：Acute paraquat poisoningAcut lung injury(ALI)Aquaporin 1(AQP1)Aquaporin 5( AQP5)
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 154-156 )