Signaling pathway regulating HSP70 in thyroxine pretreated liver ischemia/reperfusion rats
Abstract：Objective To explore the effect of thyroid hormone via PI3K-Akt pathway on heat shock protein 70 (HSP70) after liver ischemia reperfusion (I/R) injury in rats.Methods An I/R process of 30 minutes ischemia and then 48 hours reperfusion was established in female Wistar rats.The 30 rats were randomly divided into three groups:sham-operated, I/R and treatment group.Treatment of 0.1 mg/kg intraperitoneal injection of T3 at 48 hours before ischemia.The levels of MDA in liver tissue and ALT, AST and TNF-αin serum were detected at reperfusion for 48 hours.The expression of HSP70, total Akt protein and P-Akt protein were examined by Western blot.Results Compared to the I/R group, the level of ALT, AST, TNF-αin serum samples and MDA in liver tissue were lower in the other two groups ( P<0.05 ) , but they were higher in the treatment group than sham -operated group (P<0.05).The expression of HSP70 and the phosphorylation level of Akt were highest in the treatment group ( P<0.05 ) .Conclusion These results indicate that thyroid hormone up-regulates the expression of HSP70 through activation of PI3K-Akt pathway and subsequently decreases the liver ischemia reperfusion injury in rats.
Keywords：Thyroid hormoneProtein kinase BHSP70Ischemia reperfusion injuryLiver
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1036-1038,1039 )