Diammonium glycyrrhizinate down-regulating lung Toll-like receptor 4 mRNA level in acute lung injury rats induced by paraquat
Abstract：Objective To evaluate the effect of diammonium glycyrrhizinate ( DG ) on paraquat-induced acute lung injury in rats.Methods Fifty SD rats were randomly divided into control group ( NS ) , PQ group and DG group.The rats in PQ group and DG group were intragastriced administration with paraquat (100 mg/kg) .The rats in DG group were intragastriced administration with DG (50 mg/kg) once per day after paraquat injection.Pulmonary lung wet to dry weight ratio was determined;the lung tissue HE staining was used to evaluate the pulmonary tissue injury;to detect the expression of lung tissue TLR -4 mRNA and NF -κB mRNA RT -PCR method was used.The concentration of IL-6 and IL-17 in serum were determined by ELISA.Results Compared with the control group, the lung tissue's wet/dry weight ratio, expression of lung tissue TLR-4 mRNA, NF-κB mRNA, IL-6 and IL -17 in serum increased significantly and comparison between groups showed statistical significance (P<0.01 or P<0.05).7 day mortality was 90% in PQ group, 40% in DG group, and no death in NS group.Conclusion DG can attenuate the acute lung injury induced by paraquat poisoning in rats, the mechanism may be related to the decrease of TLR -4 and NF -κB mRNA expression.
Keywords：Diammonium glycyrrhizinate ( DG)Paraquat(PQ)Toll-like receptorAcute lung injury ( ALI)
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1029-1032 )