Effects of refentanil postconditioning on cell apoptosis induced by myocardial ischemia and reperfusion in rats

doi:10.3969/j.issn.1002-1949.2014.10.021

Abstract：Objective To study the effect of refentanil postcondition on cell apoptosis induced by myocardial ischemia and reperfusion in rats .Methods To build the myocardial ischemia and reperfusion injury model .The animals were randomly divided into three groups: sham operation group (sham, n=12);ischemia-reperfusion control group (I-R, n=12) and refentanil postconditioning group which was further divided into three subgroups according to the dose of refentanil ( RPO ) .In control group the hearts were subjected to 30 min ischemia followed by 90 min reperfusion ( I-R) .In RPO group the hearts were subjected to 30 min ischemia followed by 3 periods of 5 min refentanil infusion at 2 (RPO1,n=12), 4 (RPO2, n=12), 6 (RPO3, n=12) μg/kg at 5 min interval before reperfusion .Morphological change of the heart was observed by light microscopy .The apoptosis index ( AI) of cardiomyocyte was detected by TUNEL .The release of CytC protein was detected by Western blot.Results ①Light microscopy: myocardial injuries were lighter in refentanil postconditioning than those in intestinal ischemia -reperfusion group significantly .②Administration of refentanil postconditioning group exerted a significant anti-apoptotic effect, as evidenced by reduced TUNEL -positive staining .③Compared with Sham group , the expression of CytC protein in I -R and RPO group was significantly increased ( P<0.05);the expression of CytC protein in RPO group was lower than that in I-R group (P<0.05).Conclusion ①Refentanil postconditioning can protect myocardial injury induced by myocardial ischemia -reperfusion in rats .②The protect effect of refenanil is concentration dependent .③One of mechanisms of protection may be that refentanil decrease cell apoptosis .