The influence of high frequency oscillatory ventilation combined with bone marrow mesenchymal stem cell transplantation on lung fibrosis of acute lung injury

doi:10.3969/j.issn.1002-1949.2014.10.020

Abstract：Objective To investigate the influence of high frequency oscillatory ventilation combined with bone marrow mesenchymal stem cell transplantation on lung fibrosis in endotoxin -induced acute lung injury in rabbits .Methods A total of 34 rabbits were used in this study .Two rabbits were utilized to culture BMSCs and the other 32 rabbits were randomly assigned to four groups , acute lung injury group , high frequency oscillatory ventilation ( HFOV ) group, MSCs transplantation group and HFOV combined with MSCs transplantation group ( n=8 ) .Rabbit BMSCs were isolated and cultured by the whole bone marrow culture method .At the third passage , BMSCs were harvested for use . Endotoxin was infused into the trachea to establish models of acute lung injury /ARDS.Two weeks after intervention , content of lung hydroxyproline of each group were measured by acid solution;expression of transforming growth factor β( TGF-β) and monocyte chemoattractant protein -1 ( MCP-1 ) of serum and bronchoalveolar lavage fluid of each group were measured by ELISA .The lobe of right lung was excised for histological observation .Results Lung hydroxyproline content , TGF -βand MCP -1 expression in serum and bronchoalveolar lavage fluid of MSCs group were compared with acute lung injury group, the differences were statistically significant ( P <0.05 ); the difference of the single HFOV group and lung injury group was not statistically significant (P >0.05).Lung hydroxyproline content, TGF-βand MCP -1 expression in serum and bronchoalveolar lavage fluid of the HFOV +MSCs group were lowest;the differences were statistically significant (P <0.05) when compared with HFOV group and MSCs group respectively .Conclusion HFOV combined with BMSCs transplantation reduce lung hydroxyproline content , TGF -βand MCP -1 expression , and extenuate the degree of fibrosis of acute lung injury in rabbits .It has a synergistic effect that was better than a single method .