Protease-activated receptor-1 antagonist reduces the serum levels of inflammatory cytokines in post-cardiac arrest syndrome in rabbits
Abstract：Objective To observe the effect of protease -activated receptor -1 ( PAR -1 ) antagonist SCH79797 on the serum level of TNF -α, MCP -1 and IL -8 in post -cardiac arrest syndrome ( PCAS ) .Methods Thirty healthy male Japanese rabbits were divided into three groups randomly.They were the sham -operation group, PCAS group and PAR -1 antagonist group.The model of PCAS was established by asphyxia -induced cardiac arrest .Fifteen minutes after return of spontaneous circulation (ROSC), SCH79797 (25 μg/kg, iv drop, q.d.) was administrated in PAR-1 antagonist group .The indicators of organ function were evaluated at 48 hours after cardiac arrest .The serum levels of TNF-α, MCP-1 and IL-8 were determined by enzyme-linked immunosorbent assay (ELISA).Results SCH79797 alleviated the dysfunction significantly in heart , brain, liver and kidney .Compared with the sham -operation group , the serum levels of TNF-α, MCP-1 and IL-8 were increased significantly in PCAS group (P<0.01).Compared with the PCAS group, the serum levels of TNF-α, MCP -1 and IL -8 were decreased significantly in PCAS group ( P <0.05 ). Conclusion SCH79797 can reduce the serum levels of TNF -α, MCP-1 and IL-8 in post-cardiac arrest syndrome in rabbits , and attenuate multiple organ dysfunction after cardiopulmonary resuscitation .
Keywords：Protease-activated receptor -1 ( PAR-1)Post-cardiac arrest syndrome ( PCAS)Tumor necrosis factor -α( TNF-α)Monocyte chemoattractant protein-1 (MCP-1)Interleukin-8 (IL-8)
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 935-937 )