Clinical study of the dynamic changes of serum high mobility group protein B1 of Agkistrodon Halys bite patients in north China

doi:10.3969/j.issn.1002-1949.2014.10.010

Abstract：Objective To explore the dynamic changes and clinical manifestations of serum high mobility group protein B 1 of Agkistrodon Halys bite patients in north China .Methods According to clinical classification and severity grade of snake bites , we divided 87 Agkistrodon Halys bite poisoning cases into three groups:mild, severe and critical type .These patients were from Beijing or its surrounding provinces .We extracted intravenous blood of three different stage of the treatment: before treatment, two days after treatment and seven days after treatment .We also compared them to 40 healthy samples, using Enzyme -linked immunosorbent assay ( ELISA ) method to determinate the serum HMGB1 level in different periods .Finally we compared the differences between each group and analyzed the results with statistical methods .Results The serum HMGB1 levels of all the three groups of Agkistrodon Halys bite patients were higher than those normal samples (P<0.05).Serum HMGB1 in severe group was higher than that in the mild group .Serum HMGB1 in critical group was significantly higher than that in the other two groups (P<0.05).For the mild and the severe groups, the Serum HMGB1 levels after two days of treatment were significantly higher than those before treatment .The levels of serum HMGB1 after seven -day treatment were decreased significantly compared with those after tow -day treatment (P <0.05).Although decreased, the levels of HMGB1 after seven -day treatment were still higher than those before treatment (P>0.05).For the critical groups, the levels of serum HMGB1 after two-day treatment were higher than those before treatment ( P>0 .05 ) .And the levels of serum HMGB1 after seven -day treatment were still higher than those before treatment , but were not significantly lower than those of two -day treatment (P>0.05).Conclusion The levels of serum HMGB1 of Agkistrodon Halys bite patients in north China increase significantly .HMGB1 may be involved in the Agkistrodon Halys bite patient's condition occurs and development process .It can serve as a reference index which reflects the severity of poisoning .Dynamic observation of HMGB1 can guide the treatment of Agkistrodon Halys bite patients , and help determine the prognosis of patients .