An experimental study on the blood -brain barrier permeability and brain expression of ZO-1 in sepsis rats
Abstract：Obj ective To observe the changes on blood -brain barrier permeability and brain expression of ZO -1 in sepsis rats.Methods Fifty SD rats were divided into sham operation group (n=10) and model group (n =40).The model rats were further divided into 6, 12, 24, 48 h subgroups (n=10).The sepsis rat model was developed by CLP .The blood-brain barrier permeability was detected by spectrophotometric analysis of EB .The expression of ZO -1 mRNA and protein was detected by RT-PCR and Western -blot, respectively.Results The EB contents of each time point groups were higher than sham operation group (P<0.01).The expression of ZO-1 in each time point groups were lower than sham operation group (P <0.01).And the level of ZO -1 expression was continuously decreased during the development of sepsis .Conclusion The expression levels of ZO -1 mRNA and protein in sepsis rats are decreased significantly , and this may be one of the mechanisms of blood-brain barrier damage of sepsis .
Keywords：SepsisSeptic encephalopathyBlood -brain barrierZO-1Tight junction
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 775-779 )