Effect of UCF -101 on the p38 MAPK signal pathway during focal cerebral ischemia -reperfusion in rats
Abstract：Objective To investigate the neuroprotective effect and mechanisms of 5 -[5-(2-nitrophenyl)furfuryliodine]-1,3-diphenyl-2-thiobarbituric acid (UCF-101) on the cerebral neurons of rats during cerebral ischemia -reperfusion.Methods The focal cerebral ischemia models of Wistar rats were established by the middle cerebral artery occlusion ( MCAO ) with thread occlusion methods .Rats were randomly divided into sham operated group , ischemia -reperfusion group and UCF-101 treated group .24 h after focal cerebral ischemia -reperfusion , the rats were evaluated for neurological deficits and TUNEL method was used to measure apoptotic neurons , and the expression levels of p38 MAPK phosphorylation were detected by immunohistochemistry and Western blot analysis . Results UCF -101 treatment significantly improved neurological behavior and reduced TUNEL -positive cells in the cerebral cortex . Furthermore , the phosphorylation levels of p 38 MAPK were increased in ischemia-reperfusion group thanthose in sham operated group (P<0.05).In UCF-101 treated group the phosphorylation levels of p 38 MAPK was decreased compared with the ischemia -reperfusion group ( P <0 .05 ) . Conclusion p38 MAPK signal pathway might be involved in neuroprotective effect of UCF -101 treatment on focal ischemic brain of mice .
Keywords：UCF-101Cerebral ischemia-reperfusionNeuroprotectionp38 MAPKPhosphorylation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 739-742 )