Ischemic postconditioning protects both adult and aged SD rat hearts from ischemia-reperfusion injury through the PI3K/Akt and eNOS pathway
Abstract：Objective To study the protective effect of ischemic post -conditioning on rat's acute myocardial ischemia/reperfusion ( I/R) injury and the mechanism of cardiac protection through Akt and eNOS.Methods Using the Langendorff reperfusion system to establish an ex vivo rat model of whole heart I/R.Multi-conductive physiological recorder was used to record cardiac function as CF , HR, LVDP, ±dp/dtmax of all groups were recorded .Six rat hearts of each group were used to determine myocardial infarct size by dual staining with triphenyltetrazolium chloride and Evan 's blue dye. The other rats of each group were saved for Western blotting analysis as the expression of Akt , P-Akt, eNOS and P-eNOS.Results ①The cardiac function ( LVDP and ±dp/dtmax) of adult/aged IPostC group was significantly better than that of the IRI group (P<0.05).②The infarction area of adult/aged IPostC group was smaller than that of the IRI group (P<0.05).③The expression of P -Akt and P-eNOS of all adult/aged IPostC groups was significantly higher than that of the IRI groups (P<0.05). Conclusion ①Ischemic post -conditioning protects the adults or aged rats from the I /R injuries.②Given the postcondition dispose at the very beginning of the reperfusion can alleviate the acute cardiac I/R injuries through activation of PI 3K-Akt and eNOS kinase .
Keywords：Myocardial ischemia/reperfusion injuryAgedIschemic post-conditioningNOAkt
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 361-366 )