Effects of polyethylene oxide on the fluid resusciatation in septic rats

HU Zhen-hua; CHEN Zhong-qing; FANG Qin

doi:10.3969/j.issn.1002-1949.2014.04.002

Abstract：Objective To investigate the effects of polyethylene oxide ( a low-dose drag-reducing polymer ) on fluid resuscitation in septic rats .Methods A total of 48 male Wistar rats weighing 180 g to 200 g were enrolled and randomized into four groups , with 12 rats per group .The low-dose and high -dose polyethylene oxide group ( PEO1 and PEO2 group ) were treated with polyethylene oxide of 10 ppm ( parts per million ) or 50 ppm, respectively .The control group ( NS group) was treated with physiologic saline , and the blank control group ( NT group ) without fluid resuscitation.A sepsis model was established by cecal ligation and puncture (CLP) in all animals.The resuscitation was started 16 h after the operation of CLP , and the resuscitation was lasted for 4 hours. Simultaneously , the microcirculation in spinotrapezius muscle was observed under a microscope and the results were recorded .The blood pressure and heart rate were recorded .The lactic acid concentration in the blood samples was detected before resuscitation , at the end of resuscitation and 72 hours after the end of resuscitation .The survival time of each group was observed .Results The survival rate in PEO1 group was higher than the other three groups 72 h after resuscitation .The two groups treated with polyethylene oxide had a significant increase in red blood cell ( RBC) velocity of the arterioles in the spinotrapezius muscle 10 min after the beginning of resuscitation ( P <0.05 ); The RBC velocity in PEO1 group remained constant until the end of resuscitation .But the RBC velocity in PEO2 group was gradually returned to its original level in 60 min.Neither the control group nor the blank control group had a significant increase in RBC flow velocities (P>0.05).At the end of resuscitation, the RBC velocity in PEO1 group was significantly higher than that in other three groups .There was no significant change in heart rate during resuscitation in all groups (P>0.05).The blood pressure in PEO1, PEO2 and NS groups showed no significant change during resuscitation (P>0.05).But it was decreased in NT group during resuscitation (P<0.05).In addition, the lactic acid concentrations in the PEO1, PEO2 and NS groups were significantly decreased during resuscitation (P<0.05), but there was no significant change in the NT group during resuscitation ( P >0.05 ).Conclusion PEO does not significantly change the heart rate and blood pressure of the septic animals , low-dose PEO (10 ppm) can significantly increase the velocity of RBC of arteriole in spinotrapezius muscle in septic rats and improve survival rate.The high-dose PEO (50 ppm) has no improvement of the final velocity of RBC and survival rate .