Studies of Anisodamine in treatment of ALI and its effects on cytokine secretion in alveolar macrophages
Abstract：Objective To explore the mechanism of anisodamine(654-2) in the treatment of acute lung injury(ALI),the effect of 654-2 on TNF-α and IL-6 levels released by alveolar macrophages were investigated in rats.Methods ALI rats model was made by injection of lipopolysaccharide(LPS) intravenously.48 rats were equally divided into three groups:ALI model group,654-2 treatment group and normal control group.The bloods air,lung wet/dry (W/D) weight and pathological examination were made in each groups.Then the alveolar macrophages of each group were separated and cultured,the quantities of TNF-α and IL-6 were measured by bioassa methods.Results TNF-α ［(38.98±4.51)kU/L］ and IL-6［(20.82±6.3)kU/L］levels released by alveolar macrophages in 654-2 treatment group were significantly lower than those of ALI group ［TNF-α(68.27±9.13)kU/L,P＜0.001］；IL-6［（33.84±9.02)kU/L,P＜0.01］and 654-2 can also improve the blood airs levels(P＜0.05),reduce the W/D value (P＜0.05) and relieve the lung injury observed by histological examination.Conclusion 654-2 can inhibit the excessive activation and TNF-α,IL-6 secretion of alveolar macrophages,prevent the occurrance progression of ALI in rats.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 249-250 )