Experimental study on Naloxone in treating complete brain ischemia-reperfusion injury

doi:10.3969/j.issn.1002-1949.2001.04.004

Abstract：Objective To explore the protective of Naloxone hydrochloride onbrain and its mechanism during complete brain ischemia-reperfusion injury.Methods Ninety-six rats were randomized into the control group,ischemia/reperfusion injury group(IR) and Naloxone-treated group.The dynamic changes of β-EP in brain tissue and in plasma were observed.The total calcium、superoxide dismutase(SOD)、malondialdehyde(MDA)、water content of the brain tissue and the count of neurons in hippocampal CA1 were also measured.Results β-EP level in brain tissues increased remarkably(P＜0.05 and P＜0.01),the alterations of β-EP in plasma lagged it in brain tissues.The total calcium level、MDA content and water content of the brain tissues increased remarkably,the SOD activity and the count of neurons in hippocampal CA1 decreased significantly(P＜0.05 and P＜0.01),in Naloxone-treating group,the abnormal changes of indexes as above were ameliorated markedly,and there was significant difference between IR group and Naloxone group(P＜0.05 and P＜0.01).Conclusion Naloxone could attenuate complete brain ischemia-reperfusion injury,the mechanism was related to its contradicting β-EP activity and reducing the total calcium and oxygen free radicals.