Study of HSP70 protective effect on ischemic preconditioning axial flap in rats
CHEN Jin-Song
XIA Shuang-Yin
Yang Daping
Abstract：Objective This study was to reveal the continuous changes of HSP70 and its regularity in the delayed protection of ischemic preconditioning axial flap in rats,then speculate the best time- piont of protection. Methods Animals were divided into three groups randomly(control、amputate after isehemia pretreatment and amputate delay after ischemia pretreatment). A left dorsal skin flap was crated in Wistar rat to ischemia precondition model to examine the survival rate of the skin flaps, electronic microscopic appearanee, the contents of HSP70 at various times after they were given different treatment. Results The survival rate of amputate after ischamia pretreatment and amPutate delay 48 h after ischemia pretreatment are 1.26 and 1.57 times as high as that of control. The celluar biosynthesis at 48 h after IPC is vigorous when there is a lot of proteins which were synthesized and stored. The contents of HSP70 at 48 h after IPC or at 72 h after IPC is much more than that of control, especially at the 48 h. Conclusions IPC can improve the survival rate of ischemia flap and amputate delay after IPC has more effect than IPC does. 48 h may be the best time - point of IPC protection. It is suggest that the mechanism of IPC protection can be related to the function of HSP70.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 139-141 )