Study of apoptosis and Bcl - 2、 C - myc expression in ischemic and reperfusion rat myocardium

YU Hah-li; GAO Bo; LU Ying

doi:10.3969/j.issn.1002-1949.2001.03.005

Abstract：Objective To study the significance of myocyte apoptosis in myocardial ischemic reperfusion (MIR) in rats. To evaluate the relationship between Bcl- 2 and C- myc and myocyte apoptosis in MIR. Methods Myocyte apoptosis in MIR rat model was studied by TdT- mediated dUTP nick end labeling ( TUNEL). Bcl - 2 and C - myc was studied by immunobistochemical staining. Results Continuous ischemia as well as ischemia followed by reperfusion can result in myocyte apoptosis in rat myocardium. The permillage of apoptotic myocytes increased with the duration of reperfusion. We demonstrate that the fraction of cardiomyocytes undergoing apoptosis is lower in the reperfused heat than in the continuously ischemic heat. We demonstrate that the expression of Bcl - 2 is higher in the reperfused heat than in the continuously ischemic heat and the expression of C - myc is lower in the reperfused heat than in the continuously ischemic heat. Statistical analysis demonstrated a negative correlation between the pemillage of apoptotic myocytes and Bcl- 2,and a positive correlation between the permillage of apoptotic myocytes and C - myc. Conclusion The elucidation of cardiac apoptosis mechanisms will provide useful information that could potentially aid in the understanding and treatment of myocardial ischemic reperfusion injury.