SNHG11 promotion in osteosarcoma cell proliferation by activating wnt pathway
WANG Hui
MA Ming-liang
ZHANG De-gang
ZHANG Kai
JIA Long
LIU Dong
DU Gang-qiang
LI Peng
WU Hong
Abstract：Objective To investigate the function and mechanism of SNHG11 in osteosarcoma cells.Methods Ten osteosarcoma tissue specimens were collected.qRT-PCR was used to detect the expression level of SNHG11 in osteosarcoma tissues compared with matched non-tumors.CCK-8 assays were applied to test proliferation function of SNHG11 in osteosarcoma cells.The cell cycle was investigated by flow cytometry.Western blot was used to find the regulatory pathway of SNHG11.Results Compared with adjacent tissues, SNHG11 was highly expressed in osteosarcoma (n=10, t=3.16, P＜0.01).Expressions of SNHG11 in osteosarcoma cells were silenced, and the cell proliferation capacity was significantly decreased[scramble group day 1 (0.69±0.03), day 7 (2.11±0.19);SiRNA1group day 1 (0.72±0.03), day 7 (1.4±0.15);SiRNA2 group day 1 (0.67±0.07), day 7 (1.53±0.28);SiRNA-1, P＜0.01;SiRNA-2, P＜0.01].Overexpression of SNHG11 showed opposite results[NC group day 1 (0.25±0.04), day 7 (1.83±0.16);SNHG11 group day 1 (0.27±0.03), day 7 (2.55±0.23), P＜0.01].SNHG11 promoted cell proliferation by inducing the progression of osteosarcoma cell cycle, and it accelerated the proliferation of osteosarcoma cells by activating the WNT signaling pathway.Conclusions SNHG11 high expression in osteosarcoma tissues accelerates osteosarcoma cell growth by activating wnt pathway.
Keywords：SNHG11Wnt signaling pathwayOsteosarcomaCell proliferation
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 66-70 )