Effects of ginsenoside Rb2 on osteoclast regulation in vitro
SU Ke
QIU Yu-cheng
WANG Chun-mei
YUAN Zhi
Abstract：Objective To investigate effects and mechanism of ginsenoside Rb2 on osteoclasts in vitro. Methods The mouse osteoclast precursor cells RAW 264.7 were cultivated to induce osteoclasts. Rb2 ( 0.1 μM, 1 μM, 10 μM ) was respectively added in the culture solution. CCK-8 was used for the detection of drug toxicity. The morphology and count of osteoclasts were observed by TRAP staining. The expression of osteoclast specific gene mRNA, such as TRAP and NFATc1, was detected by RT-PCR. Western blot technique was used to detect the expression levels of LC3 I, LC3 II and mTOR proteins. Results Compared with the control group ( 168.2 ± 22.6 ), the number of osteoclasts decreased significantly: group 0.1 μM ( 131.0 ± 16.3 ), P = 0.018; group 1 μM ( 98.8 ± 17.9 ), P<0.01;group 10 μM(85.8 ± 17.3),P<0.01.The expression of TRAP and NFATc1 osteoclast specific gene mRNA decreased significantly, the ratio of LC3, II / LC3 and I increased significantly, and the amount of mTOR decreased, P< 0.01. Conclusions Rb2 may affects the expression of osteoclast genes by autophagy pathway to reduce the osteoclast formation. mTOR pathway plays an important role in this process.
Keywords：GinsenosidesOsteoclastsAutophagyOsteoporosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 874-877 )