Research on the injectable demineralized bone matrix for the calvarial bone defect in a rat model
LI Mao
SHEN Ya-jun
ZHAO Yan-tao
HOU Shu-xun
Abstract：Objective To obverse and evaluate the physicochemical properties and ability to repair bone defects of injectable demineralized bone matrix ( DBM ) prepared by a modified decalcification technique. Methods The DBM was prepared by the modified dynamic decalcification and double circle acid treatment method. The surface topography and composition were observed through a scanning electron microscope ( SEM ). And a continuum-source atomic absorption spectrometry ( CS-AAS ) was used to determine the samples' calcium content. The injectable DBM was prepared from DBM granules by using the excipient. We implanted the DBM 1 ( the traditional method ) and DBM 2 ( the modified dynamic decalcification and double circle acid treatment method ) into the rat calvarial defect model. A blank control group was set. Rats were euthanised at 4 and 8 weeks after the operation. The repair effects of rat calvarial defects were evaluated by micro-computed tomography and histological analyses, with quantitative comparison of the new bone. Results There were no obvious differences in the surface topography and composition between the DBM 1 and DBM 2. The calcium content was significantly lower than the standard value. The micro-computed tomography analyses consistently revealed that the bone volume of newly formed bone ( BV-NFB ) and the percentage of the BV-NFB were ( 4.6 ± 0.8 ) mm3, ( 30.5 ± 5.3 ) % ( 4 w ) and ( 7.6 ± 1.4 ) mm3, ( 51.4 ± 9.5 ) % ( 8 w ) in the DBM 2 group; ( 3.6 ± 0.6 ) mm3, ( 23.8 ± 4.0 ) % ( 4 w ) and ( 6.2 ± 0.9 ) mm3, ( 41.9 ± 6.1 ) % ( 8 w ) in the DBM 1 group; ( 1.3 ± 0.4 ) mm3, ( 8.6 ± 2.6 ) % ( 4 w ) and ( 2.1 ± 0.8 ) mm3, ( 14.2 ± 5.4 ) % ( 8 w ) in the blank control group. The DBM 2 group was superior to both the DBM 1 group and the blank control group ( P < 0.05 ). The histological analyses at 8 w after the operation indicated the more new bone formed in the DBM 2 group than the DBM 1 group and the blank control group. Conclusions The DBM 2 prepared by the modified dynamic decalcification and double circle acid treatment method shows excellent osteogenic capacity when compared with the DBM 1 prepared by the traditional method, which takes a shorter time and is conducive to industrial production.
Keywords：Decalcification techniqueRatsSkullDemineralized bone matrix
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 283-288 )