Research on the influence of joint injuries on gene expressions of articular cartilage proteases
XUE Jian-li
CUI Wei
Abstract：Objective To explore whether injuries of the knee joint tissues will increase gene expressions of selected hyaline cartilage degenerating enzymes such as matrix metaloproteinases ( MMP ) and aggreacaneses ( Agg ). Methods A total of 138 patients with joint tissue lesions such as menisci, anterior cruciate ligament ( ACL ) and hyaline cartilage were admitted for knee arthroscopy. There were 81 females and 57 males with a mean age of 38.8 years. Full blood samples were collected preoperatively and synovium samples intraoperatively. Real time polymerase chain reaction ( PCR ) and spectrophotometric analysis were performed. Results ACL lesions were found in 56 patients, medial menisci ( MM ) lesions in 65 patients and lateral menisci ( LM ) lesions in 5 patients. Chondral lesions were estimated according to Outerbridge’s grading system. In laboratory tests, significant correlation was seen between ACL tears and gene expressions ( MMP1, MMP2, MMP8, MMP9, MMP13, MMP14, Agg1, Agg2, IL1, TNFαand TIMP2, except TIMP1 ) ( P < 0.05 ). The gene expression level in synovium of the patients with ACL lesions was significantly elevated compared with that of the patients without ACL lesions: MMP1 0.920 ± 0.068 vs 0.794 ± 0.061;MMP2 1.075 ± 0.053 vs 0.668 ± 0.035; MMP8 0.951 ± 0.047 vs 0.766 ± 0.045; MMP9 1.354 ± 0.032 vs 0.947 ± 0.042;MMP13 1.148 ± 0.058 vs 0.991 ± 0.058; MMP14 1.379 ± 0.049 vs 0.777 ± 0.036; Agg1 1.309 ± 0.071 vs 0.647 ± 0.034; Agg2 1.043 ± 0.072 vs 0.684 ± 0.069; IL1 1.320 ± 0.054 vs 0.857 ± 0.049; TNFα 1.101 ± 0.050 vs 0.802 ±0.039; TIMP1 1.197 ± 0.060 vs 1.035 ± 0.071; TIMP2 1.110 ± 0.048 vs 0.861 ± 0.048 ( P < 0.05 ). Except TIMP1 ( 1.092 ± 0.053 vs 1.081 ± 0.033, P = 0.32 ), the gene expression level in serum of the patients with ACL lesions was significantly elevated compared with that of the patients without ACL lesions: MMP1 1.163 ± 0.030 vs 0.948 ± 0.049;MMP2 0.918 ± 0.056 vs 0.827 ± 0.049; MMP8 1.172 ± 0.029 vs 0.783 ± 0.034; MMP9 1.304 ± 0.059 vs 0.94 ± 0.04;MMP13 1.155 ± 0.060 vs 0.824 ± 0.043; MMP14 1.095 ± 0.058 vs 0.759 ± 0.033; Agg1 1.270 ± 0.077 vs 0.676 ± 0.038; Agg2 1.244 ± 0.037 vs 0.871 ± 0.037; IL1 1.128 ± 0.056 vs 0.912 ± 0.045; TNFα 1.018 ± 0.076 vs 0.724 ± 0.047;TIMP1 1.092 ± 0.053 vs 1.081 ± 0.033; TIMP2 0.906 ± 0.056 vs 0.875 ± 0.049 ( P < 0.05 ). All of the gene expressions were correlated in the serum and synovium ( Agg1: r = 0.89, MMP1: r = 0.88, MMP2: r = 0.87, P < 0.001 ). Conclusions An ACL lesion provokes elevation in the gene expression of proteases, while the influence of other lesions remains elusive. Gene expression in the synovium is correlated with that in the peripheral blood.
Keywords：OsteoarthritiskneeHyaline cartilageMetalloendopeptidasesAnterior cruciate ligamentMeniscitibial
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 915-919 )